human fetal small intestinal epithelial fhs 74 int cell line  (ATCC)

Journal: The Korean Journal of Parasitology

Article Title: Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line

doi: 10.3347/kjp.2021.59.6.573

Figure Lengend Snippet: Toxoplasma gondii and ESP induced cytotoxicity and apoptosis in human fetal small intestinal epithelial FHs 74 Int cells. FHs 74 Int cells were stimulated with the T. gondii RH strain at a multiplicity of infection (MOI) of 10 or with 1 μg/ml ESP for 0, 2, 8, and 24 hr. (A) The lactate dehydrogenase (LDH) level in the medium. The horizontal lines in each group represent mean±SD. *** P <0.001, compared to the untreated control cells. (B) Cleaved PARP and cleaved caspase 3 protein levels. (C, D) Expression of cytochrome c of in mitochondrial and cytosolic fractions.

Article Snippet: A nontransformed human fetal small intestinal epithelial cell-line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, Virginia, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), antibiotic-antimycotic (Gibco BRL) and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, New York, USA) at 37°C in a humidified atmosphere with 5% CO 2 .

Techniques: Infection, Control, Expressing

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Expression of nucleotide-binding oligomerization domain-like receptors (NLRs), inflammasome components, and caspase-cleaved interleukins (ILs) in human small intestinal epithelial (FHs 74 Int) cells. Total RNA isolated from the untreated cells was examined by polymerase chain reaction (PCR) for mRNA expression of different genes. a Expression of NLR mRNAs in FHs 74 Int cells. b Expression of the NLR mRNAs in FHs 74 Int cells compared to HPRT-1. c Positive controls for primer functionality using human acute monocytic leukemia cell line (THP-1). d Inflammasome components and caspase-cleaved ILs expressed in FHs 74 Int cells. Images shown are representatives of five independent experiments

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Expressing, Binding Assay, Isolation, Polymerase Chain Reaction

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Expression of NLR family members, NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome components in response to T. gondii infection in human small intestinal epithelial (FHs 74 Int) cells. a FHs 74 Int cells were infected with T. gondii at a MOI of 10 for 0, 4, and 8 h. Expression of NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome component proteins were detected by western blot analysis; α-tubulin was used as the loading control. b FHs 74 Int cells were fixed and probed against cleaved caspase-8 (green). The cells were counterstained with 4′,6-diamidino-2-phenylindole (blue) and visualized using confocal microscopy. All data shown are representative of three independent experiments with similar results

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Expressing, Infection, Western Blot, Control, Confocal Microscopy

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Schematic model of NLRP3 inflammasome activation in T. gondii -infected FHs 74 Int cells. T. gondii activates the p38 MAPK pathway in small intestinal epithelial cells, subsequently upregulating protein expression and promoting the formation of the NLRP3 inflammasome. The NLRP3 inflammasome cleaves pro-IL-1β, pro-IL-18 and pro-IL-33 to become active IL-1β, IL-18, and IL-33, which ultimately induces cytotoxicity of FHs 74 Int cells

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Activation Assay, Infection, Expressing